Regulatory
G41

Part:BBa_J119413:Design

Designed by: Isabella Wiebelt-Smith   Group: Eckdahl Lab   (2018-11-07)


G41 - i-motif sequence on pTac coding strand


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The lengths of the loops in the i-motif had to be shortened so that the sequence would fit within the parameters of the promoter's -35 and -10 elements with a loop at the -10 region.


Source

pTac is a strong promoter sequence that combines pLac and p____. The i-motif-forming sequence is a modified version of the Nuclease Hypersensitive Element III of the human c-Myc promoter.

References